ORIGINAL ARTICLE

 

Evaluation of cytotoxicity of accessories used for traction of impacted teeth

 

 

Rogério Lacerda dos Santos^I; Matheus Melo Pithon^I; Fernanda Otaviano Martins^II; Maria Teresa Villela Romanos^III; Antônio Carlos de Oliveira Ruellas^IV

^IDDS, MS, PhD Student in Orthodontics, Department of Orthodontics Federal University of Rio de Janeiro, Brazil
^IIBS, Student in Microbiology, Department of Microbiology, Orthodontics Federal University of Rio de Janeiro, Brazil
^IIIBS, Assistant Professor of Microbiology, Department of Microbiology, Federal University of Rio de Janeiro, Brazil
^IVDDS, MS, PhD, Assistant Professor of Orthodontics, Department of Orthodontics, Federal University of Rio de Janeiro, Brazil

Correspondence to

 

 

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ABSTRACT

AIM: To test the hypothesis that gold-coated orthodontic accessories used for canine traction are less cytotoxic than those made of stainless steel.
METHODS: Six different orthodontic accessories were evaluated, three of them made from stainless steel (1 – bracket, 2 – button, 3 – mesh pad) and three made from a gold-coated alloy (4 – small mesh pad, 5 button, 6 – big mesh pad). Three control groups were also analyzed: Positive control (C+), consisting of Tween 80 cell detergent; Negative control (C-), consisting of PBS; and Cell control (CC), consisting of cells not exposed to any material. Dye-uptake technique, in which neutral red dye is incorporated into viable cells, was used to assess the cytotoxicity of the accessories. Viable cell counting was performed using a spectrophotometer. Data were analyzed statistically by ANOVA and Tukey’s test.
RESULTS: Statistically significant differences (P< 0.05) were found between Groups 1-3 and Groups 4-6. However, no differences were found between Groups 1-3 and Groups C- and CC, and neither between Groups 4-6 and Group C+.
CONCLUSIONS: The tested hypothesis was not confirmed since gold-coated orthodontic accessories were found to be more cytotoxic than those made of stainless steel.

Keywords: tooth, impacted, orthodontic appliance, orthodontic appliance design.

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Correspondence to:
Rogério Lacerda dos Santos
Rua Ipatinga, 170, Planalto
Divinópolis- MG- Brasil - CEP: 35501-191
E-mail: lacerdaorto@hotmail.com

Received for publication: December 16, 2009
Accepted: June 21, 2010