MOTA, Ana Carolina Costa da et al. Cytotoxic effect of glass ionomer cements in cell culture. Rev. Assoc. Paul. Cir. Dent. []. 2014, 68, 2, pp. 166-169. ISSN 0004-5276.

Aim: The aim of the present study was to assess the toxicity of different chemically activated GICs in fibroblast cell cultures post-agglutination (PA) and post-setting reaction (PS). Material and Methods: The materials were divided into nine experimental groups: Group I - control; Group II (PA) - Riva Self Cure (SDI); Group III (PS) - Riva Self Cure (SDI); Group IV (PA) - Maxxion R (FGM); Group V (PS) - Maxxion (FGM); Group VI (PA) - Vitro Molar (DFL); Group VII (PS) - Vitro Molar (DFL); VIII (PA) - Ketac Molar Easymix; and Group IX (PS) - Ketac Molar Easymix (3M/ ESPE). The cements were mixed following the manufacturers' instructions, deposited on glass slip covers and placed into contact with the cell cultures immediately following the agglutination of the material or after the setting reaction. The effect of the materials on the cultivated fibroblasts was analyzed in terms of short-term and long-term cell growth as well as in viability and cell proliferation assays. Results: When placed in contact with the cells immediately following agglutination, the materials caused immediate cell death. The groups having received the materials following the setting reaction had a lower number and percentage of viable cells in comparison to the control, but the materials allowed cell growth throughout the experiment. KetacMolar Easymix (3M/ESPE) was the glass ionomer cement with the least amount of cytotoxic activity on fibroblasts. Conclusion: All glass ionomer cements tested were toxic prior to the setting reaction.However after setting reaction, allowed cell growth.

: glass ionomer cements; cytotoxicity tests; immunologic; cell culture techniques.