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Revista Brasileira de Odontologia

versão On-line ISSN 1984-3747versão impressa ISSN 0034-7272

Resumo

MARINS, Fernanda Cunha et al. Cytotoxicity evaluation of two MTA-based root canal sealers: an in vitro study. Rev. Bras. Odontol. [online]. 2017, vol.74, n.1, pp. 27-30. ISSN 1984-3747.

Objective: this study evaluated the cytotoxic effects of two MTA-based root canal sealers (Endoseal and MTA Fillapex) on human MRC-5 fibroblasts by comparing cell viability with an epoxy resin-based sealer (AH Plus) and negative control. Material and Methods: MRC-5 human fibroblasts were incubated with four different elutes of Endoseal, MTA Fillapex, and AH Plus for 24 hours. The cytotoxicity of tested materials was determined using the MTT assay. The results were analyzed using ANOVA and Tukey tests using SPSS software 15.0 (SPSS Inc, Chicago, IL). The level of significance was P < 0.05. Results: at 1:1 dilution, there was no statistical difference between all root canal sealers tested (P > 0.05). AH Plus showed significantly more cell viability (mitochondrial activity of cells) than Endoseal and MTA Fillapex at 1:2 dilution (P < 0.05). At 1:4 and 1:8 dilutions, MTA Fillapex showed less cell viability than AH Plus and EndoSeal (P < 0.05). At these same dilutions, no statistically significant differences were observed between AH Plus and EndoSeal (P > 0.05). Conclusion: the cytotoxicity effects of all endodontic root canal sealers were dose-dependent. Endoseal showed significantly more cell viability than MTA Fillapex in two of four dilutions tested. AH Plus showed statistically lower cytotoxicity than the two MTA-based root canal sealers in one of four dilutions evaluated.

Palavras-chave : Root canal Filling Materials; Periodontal Ligament; Cytotoxicity..

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