Resumo

MAYER, Luciano et al. Methodology protocol for assess systemic and local effects of LLLT on osseointegration of dental implants in the rabbits jaws. RFO UPF [online]. 2013, vol.18, n.2, pp. 235-245. ISSN 1413-4012.

Objectives: to describe surgical and laboratorial techniques used in an experimental rabbit model. The aim of the study was to obtain data using the following techniques: hormonal dosage, resonance frequency analysis (RFA), cone beam computed tomography (CBCT), scanning electron microscopy (SEM), energy dispersive spectrometry (EDS), peri-implant histological and histomorphometric analysis after low-level laser therapy (LLLT). Materials and method: a total of 40 New Zealand male rabbits were divided into five groups. Two groups designated control (CI and CII) and three groups designated experimental (EI, EII, EIII). All groups underwent blood sampling for measurements of triiodothyronine (T3) and thyroxine (T4), calcium and albumin, with the CI group being considered absolute hematological control. For the other groups (CII, EI, EII and EIII) the extraction of the lower left incisor was performed followed by placement of an immediate implant with measurement of resonance frequency. The experimental groups were subjected to laser therapy with three different doses (EI = 70J/cm2, EII = 35J/cm2, EIII = 140J/cm2). Groups CII, EI, EII and EIII were subjected to the measurement of resonance frequency after 45 days, killed and the jaw was dissected to be evaluated by CBCT, SEM and EDS. Then, the samples were included, sectioned and stained with HE staining, picrosirius-red and toluidine blue for histomorphometric analysis by linear extent of bone-implant contact (BIC) and bone area (BA) using light microscopy. Final considerations: a technical sequence that can provide a model for fellow researchers was presented.

Palavras-chave : Dental implants.; Low-level laser therapy.; Methodology.; Rabbits..