Resumo

CARDOSO, Andreia Medeiros Rodrigues; CAVALCANTI, Yuri Wanderley; ALMEIDA, Leopoldina Fátima de  e  PADILHA, Wilton Wilney Nascimento. Antibacterial activity of plant-based tinctures on dental biofilm's microrganisms. Odontol. Clín.-Cient. (Online) [online]. 2012, vol.11, n.3, pp. 212-214. ISSN 1677-3888.

Objective: The aim was to evaluate the Minimum Inhibitory Concentration (MIC) of tinctures from Salvia - T1 (Salvia officinalis), Malva - T2 (Malva sylvestris) and Purple Cashew - T3 (Anacardium occidentale) against Streptococcus mutans (ATCC 25175) and Streptococcus oralis (ATCC 10557). Material and Method: The MIC of tinctures was determined through the microdilution technique using 96-wells microplates. To each well were placed 100mL of BHI doubly concentrated, 100μL of the tinctures in concentrations that ranged from 100 to 0.78 mg/mL and 10 mL of microbial inoculum (1.5x108 UFC/mL). The microplates were incubated at 37°C for 24 hours. The MIC corresponded to the last dilution in which there was no presence of bacterial precipitate or turbidity in the culture medium. Chlorhexidine at 0,12% (1,2mg/mL) was used as control. Results: For S. mutans and S. oralis the MIC for each tincture were respectively 3.12 and 0.78 (T1), 6.25 and 3.12 (T2), 1.56 and 0.78 (T3). Chlorhexidine 0.12% presented satisfactory activity against the studied strains. Conclusion: The analyzed tinctures presented antibacterial activity against the evaluated strains of Streptococcus.

Palavras-chave : Microbial Sensitivity Tests; Plant Preparations; Dental Plaque..