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Revista Odonto Ciência (Online)

  ISSN 1980-6523

CARVALHO, Suzana Papile Maciel; SALES-PERES, Arsenio; RIBEIRO-BICUDO, Lucilene Arilho    SILVA, Ricardo Henrique Alves da. Quality evaluation of DNA obtained from stored human saliva and its applicability to identification in Forensic Dentistry. Rev. odonto ciênc. (Online) []. 2010, 25, 1, pp. 48-53. ISSN 1980-6523.

PURPOSE: This study evaluated the quality of DNA obtained from stored human saliva and its applicability to human identification. METHODS: The saliva samples of 20 subjects, collected in the form of saliva in natura and from mouth swabs and stored at -20ºC, were analyzed. After 7 days, the DNA was extracted from the 40 saliva samples and subjected to PCR and electrophoresis. After 180 days, the technique was repeated with the 20 swab samples. RESULTS: The first-stage results indicated that DNA was successfully extracted in 97.5% of reactions, 95% of saliva in natura and 100% of swab saliva samples, with no statistically significant difference between the forms of saliva. In the second phase, the result was positive for all 20 analyzed samples (100%). Subsequently, in order to analyze the quality of the DNA obtained from human saliva, the SIX3-2 gene was tested on the 20 mouth swab samples, and the PCR products were digested using the MbO1 restriction enzyme to evaluate polymorphisms in the ADRA-2 gene, with positive results for most samples. CONCLUSION: It was concluded that the quantity and quality of DNA from saliva and the techniques employed are adequate for forensic analysis of DNA.

: Human identification; Forensic Dentistry; Forensic Medicine; DNA; molecular biology; saliva.

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