Resumo

VIEIRA, Thiago Isidro; GONDIM, Brenna Louise Cavalcanti; SANTIAGO, Bianca Marques  e  VALENCA, Ana Maria Gondim. In vitro antibacterial and non-stick activity of extracts from leaves of Psidium guineense Sw. and Syzygium cumini (L.) Skeels on oral microorganisms. RGO, Rev. gaúch. odontol. (Online) [online]. 2012, vol.60, n.3, pp. 359-365. ISSN 1981-8637.

Objective This study determined the Minimum Inhibitory Concentration and the Minimum Inhibitory Concentration of Adherence of hydroalcoholic extracts of the leaves of strawberry guava (Psidium guineense Sw.) and of the jambolan (Syzygium cumini (L.) Skeels) against Streptococcus mutans (ATCC 25175), Streptococcus oralis (ATCC 10557), Streptococcus parasanguis (ATCC 903), Streptococcus salivarius (ATCC 7073), Streptococcus sp (ATCC 15300), and Lactobacillus casei (ATCC 9595). Methods Strains were seeded on blood agar plates to determine the Minimum Inhibitory Concentration by the agar-diffusion technique. The inclined tubes technique was used for Minimum Inhibitory Concentration of Adherence evaluation in the presence of 5% of sucrose, in Mueller-Hinton broth. The same procedures were accomplished with the 0.12% chlorhexidine digluconate (positive control). Assays were performed in duplicate. The plates and the tubes were maintained in microaerophillia at 37ºC for 24 hours. Results The Minimum Inhibitory Concentration obtained for strawberry guava extract ranged from 275 to 1100 mg.ml-1. Jambolan values were 242.5 to 485 mg.mL-1; and 0.12% chlorhexidine digluconate were 75x10-3 to 9x10-3 mg.ml-1. The Minimum Inhibitory Concentration of Adherence reported the following values : strawberry guava (1.81 to 28.94 mg.ml-1); jambolan (1.60 to 12.76 mg.ml-1) and 0.12% chlorhexidine didigluconate (4.93 x10-4 to 19.70 x10-4 mg.ml-1). Conclusion It was concluded that the hydroalcoholic extracts from the leaves of P. guineense Sw. and S. cumini (L.) Skeels presented antimicrobial and nonstick effect on the tested lineages; further studies are needed to confirm these extracts to be natural antibacterial agents for use in controlling dental caries.

Palavras-chave : Anti-bacterial agents.; Biofilms.; Phytotherapy.; Psidium.; Syzygium jambolanum..