Resumo

ENNES, Jussara Peixoto et al. Immunolocalization of FGF-10 in murine incisors and molar germs. RGO, Rev. gaúch. odontol. (Online) [online]. 2012, vol.60, n.4, pp. 497-502. ISSN 1981-8637.

Objective The aim of this study was to identify the presence of FGF-10 in mouse dental germs by means of the immunohistochemical technique, from the initial development phase through to the more advanced phases. Methods Fetuses of five mice, on days 15.5, 16.5, 17.5, 18.5 and 19.5 of pregnancy, respectively, were collected. At time intervals of 0.5, 1.5, 2.5 and 3.5 days after birth, the mouse offspring were sacrificed. The heads of all the specimens were fixed and submitted to histotechnique and 3μm thick sections were obtained. The presence of FGF-10 was detected by means of the avidin-biotin-peroxidase immunohistochemistry technique. Results Immunostaining was detected in both epithelium and ectomesenchyme with intensity and spatial-temporal differences. A reduction in the presence of FGF-10 was observed in the cervical loop area, on the lingual side of the incisor crowns, and both sides of the molar crowns. With the increase in enamel matrix deposition, immunostaining on the secretory pole of ameloblasts also increased. Conclusion FGF-10 immunostaining could be related to cell proliferation in epithelium and cell differentiation in epithelium and ectomesenchyme. This could be related to morphological determination in intercuspal areas of molar germs and to continuous growth of the incisor crown. Decrease in FGF-10 in the cervical loop could be related to the termination of crown formation. Increase in FGF-10 expression in ameloblasts suggests a relationship with active enamel production. The results suggest the inclusion of the pattern of the presence of FGF-10 in future investigations into the cause of morphological anomalies, such as palato-gingival groove.

Palavras-chave : Immunohistochemistry.; Odontogenesis.; Tooth development..