Resumo

ROMEIRO, Rogério de Lima et al. Adhesion of microorganisms in dental implants with a smooth surface or treated. Innov. Implant. J., Biomater. Esthet. (Online) [online]. 2010, vol.5, n.2, pp. 25-29. ISSN 1984-5960.

The aim of this study was to analyze in vitro the adherence of Streptococcus sanguinis to dental implant surfaces treated with calcium phosphate blasting, anodizing, double acid etching and smooth surface. We selected 40 implants, 10 in each area. For analysis of adhesion of microorganism suspensions were prepared containing 106 cells/ml in a spectrophotometer. The implant was removed from its packaging and placed directly in the broth. Then were placed separately in of culture plates of cells containing broth containing sucrose (plate in vitro) and suspension of the microorganism. After 24h incubation at 37 oC and 5% CO2, the implants were washed three times for one minute in sterile saline and placed in a sonicator with 10 ml of saline for dispersion of the adhered cells. Next, serial dilutions were performed sowing and in culture media specific for each microorganism. After 48 h incubation at 37 oC and 5% CO2, were the count of colony forming units (CFU/ml) and the data were subjected to analysis of variance (ANOVA), Tukey test, with significance level 5%. The results showed a high adherence of microorganisms to surfaces studied. The anodized surface had the lowest values of adherence of two microorganisms, since the surface subjected to the double acid etching presented higher values of CFU/ml.

Palavras-chave : Dental implants; Streptococcus sanguis; Periodontics.