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Brazilian Journal of Oral Sciences

versão On-line ISSN 1677-3225

Braz. J. Oral Sci. vol.9 no.2 Piracicaba Abr./Jun. 2010




Longitudinal evaluation of the effect of saliva contamination during the bonding protocol with a self-etch adhesive system



Samuel Nilo VieiraI; Fernando Aparecido KawaguchiII; Sergio Brossi BottaIII; Adriana Bona MatosIV

IDDS, Undergraduate student, Department of Operative Dentistry, Dental School, University of São Paulo, Brazil
IIDDS, MS, PhD, Graduate student, Department of Operative Dentistry, Dental School, University of São Paulo, Brazil
IIIDDS, MS, Graduate student, Department of Operative Dentistry, Dental School, University of São Paulo, Brazil
IVDDS, MS, PhD, Professor, Department of Operative Dentistry, Dental School, University of São Paulo, Brazil

Correspondence to




AIM: To evaluate the influence of saliva contamination on the short- and long-term bond strength of a self-etch adhesive system.
One hundred and twelve non-carious human molars were randomly divided according to: substrate (enamel/dentin); presence of saliva [none (control- C), before primer (BP), after primer (AP) and after bonding agent (AB)]; treatment of the contamination [none (1), rinsing + drying (2), drying (3) and primer re-application (4)] and
specimen storage (24 h or 6 months). A self-etch adhesive system was applied to the dental surfaces followed by incremental insertions of composite resin. After storage in water at 37oC, the specimens were perpendicularly cut into beams for microtensile bond strength testing. Data in MPa were compared by ANOVA followed by Tukey’s test (p< 0.05). Micrographs were obtained by low vacuum scanning electron microscopy.
Control groups (G1 and G8) presented
higher bond strength than all other groups. The factors presence of saliva, treatments of the contaminant and specimen storage showed no statistically significant results for the two dental substrates. Contaminants could be detected by LV-SEM. Six-month storage did not affect bond strength.
The presence of saliva during the application of the self-etch system was deleterious to the bond to enamel and dentin, irrespective of the operative step in which the contamination occurred.

Keywords: saliva contamination, self-etch adhesive, bond strength, longevity.



Full text available only in PDF format.




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Correspondence to:
Adriana Bona Matos
Av. Prof. Lineu Prestes, 2227
Cidade Universitária
CEP: 05508-000, São Paulo, SP, Brasil
Phone: 55-11-30917839 ext. 224 - Fax: 55-11-37334307

Received for publication: November 21, 2009
Accepted: June 08, 2010