ORIGINAL ARTICLE

 

Hyaluronan does not improve bone healing in critical size calvarial defects in rats - a radiographic evaluation

 

 

Mariana Amade Mendes Brazão^I, Beatriz de Brito Bezerra^II, Márcio Zaffalon Casati^III, Enilson Antônio Sallum^III, Antônio Wilson Sallum^IV

^IDDS, Graduate Student, Department of Endodontics, São Leopoldo Mandic Dental School
^IIDDS, MS. Graduate Student, Department of Prosthodontics and Periodontics, Division of Periodontics, Piracicaba Dental School, State University of Campinas
^III DDS, MS, PhD. Professor, Department of Prosthodontics and Periodontics, Division of Periodontics, Piracicaba Dental School, State University of Campinas
^IVDDS, PhD, Professor, Department of Prosthodontics and Periodontics, Division of Periodontics, Piracicaba Dental School, State University of Campinas

Correspondence to

 

 

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ABSTRACT

AIM: This study evaluated radiographically the effects of 1% hyaluronan in bone healing using a critical size rat-calvaria defect model.
METHODS: Thirty adult male Wistar rats were used in this study. Two 6-mm-diameter critical-size defects were created and the treatments were randomly distributed as follows: 1) 1% hyaluronan; 2) 1% hyaluronan soak loaded onto an absorbable collagen sponge (ACS) carrier; 3) saline; and 4) ACS alone. The animals were sacrificed at 4 and 8 weeks when biopsies were collected and radiographs obtained using a direct digital radiograph system and a standardized protocol. A blind examiner evaluated the radiographic density of the images twice and an intraclass correlation was performed to evaluate examiner reproducibility (R2=0.99, p<0.001). Comparisons between 4 and 8 weeks of treatment were performed by Student’s t test and comparisons between treatments and time by two-way ANOVA at 5% significance level.
RESULTS: There were no noteworthy differences between 4 or 8 weeks within each treatment group (p>0.05). When treatments were compared no significant differences between groups were found (p>0.05).
CONCLUSIONS: Within the limits of this study, it can be concluded that 1% hyaluronan gel alone or its association with a carrier does not improve bone healing.

Keywords: bone repair, wound healing, hyaluronan.

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Correspondence to:
Beatriz de Brito Bezerra
Departmento de Prótese e Periodontia,
Divisão de Periodontia - FOP/UNICAMP
Av. Limeira, 901 – 13.414-903
Caixa postal 52 - Piracicaba, Brazil
Phone: +55 19 2106-5301
E-mail: beatrizbb@yahoo.com

Received for publication: March 02, 2010
Accepted: June 01, 2010