ORIGINAL ARTICLE

 

Cytotoxicity of orthodontic elastics: In vitro investigation with on L929 mouse fibroblasts

 

 

Rogério Lacerda dos Santos^I; Matheus Melo Pithon^I; Fernanda Otaviano Martins^II; Maria Teresa Villela Romanos^III

^ISpecialist in Orthodontics, Federal University of Alfenas, Minas Gerais, Brazil. Master in Orthodontics, Federal University of Rio de Janeiro, Brazil. PhD student in Orthodontics, Federal University of Rio de Janeiro, Brazil
^IIGraduated in Biological Sciences: Microbiology and Immunology Federal University of Rio de Janeiro-UFRJ
^IIIAssistant Professor of Microbiology, Federal University of Rio de Janeiro, Brazil

Correspondence to

 

 

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ABSTRACT

AIM: To test the hypothesis that there is no difference in the cytotoxicity among natural latex elastics of different manufacturers using a L929 cell line culture.
METHODS: Different latex intraoral elastics (I.D. = 5/16", 4.5 oz.) were tested. The sample was divided into 7 groups of 15 elastics each: Group AO (American Orthodontics), Group GAC (GAC International), Group TP (TP Orthodontics), Group AD (Aditek), Group AB (Abzil), Group MO (Morelli) and Group UN (Uniden). Cytotoxicity assays were performed by using cell culture medium containing L-929 line cells (mouse fibroblast). The cytotoxicity was evaluated by using the "dye-uptake" test, which was employed at two different moments (1 and 24 h). Data were compared by ANOVA and Tukey's test (P < 0.05).
RESULTS: The results showed a significant difference (P < 0.05) between all groups and the group CC (cell control) at 1 and 24 h. Groups AD, AB, MO and UN were noticeably more cytotoxic than the groups AO, GAC and TP at 1 h. After 24 h, a significant decrease in cell viability was observed in all groups.
CONCLUSIONS: Intraoral elastics from American Orthodontics, GAC and TP Orthodontics trademarks induced less cell lysis than Aditek, Abzil, Morelli and Uniden trademarks.

Keywords: cytotoxicity, elastics, biocompatibility, orthodontics.

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Correspondence to:
Rogério Lacerda dos Santos
Rua Ipatinga, 170, Planalto
Divinópolis- MG- Brazil CEP 35501-191
E-mail: lacerdaorto@hotmail.com; lacerdaorto@bol.com.br

Received for publication: April 08, 2010
Accepted: August 23, 2010