SciELO - Scientific Electronic Library Online

 
vol.11 número2 índice de autoresíndice de assuntospesquisa de artigos
Home Pagelista alfabética de periódicos  

Brazilian Journal of Oral Sciences

versão On-line ISSN 1677-3225

Braz. J. Oral Sci. vol.11 no.2 Piracicaba Abr./Jun. 2012

 

ORIGINAL ARTICLE

 

Aging and thermocycling effects on adhesion of fiber posts to human radicular dentin

 

 

Abu-Naba'a LAI; Abu-Rujai MII

 

IBDS, PhD, MFDRCS, Assistant Professor of Prosthodontics, Department of substitutive oral sciences, Taibah University, KSA, Formerly: Jordan University of Science and Technology, Jordan
IIMaster's degree student, Department of Prosthodontics, Jordan University of Science and Technology, Irbid, Jordan

Correspondence

 

 


ABSTRACT

AIM: This in vitro study evaluated the effect of different aging periods and thermocycling on the adhesion of fiber posts to human dentin at different depths.
METHODS: Twenty teeth were cleaned, decoronated, and endodontically treated. After one week, root filling was removed and a 10-mm post space was prepared. Posts (RelyX™ Fiber size 2) were cemented with a self-adhesive resin cement (RelyX™ Unicem) and light cured. Specimens (n=4, each) were stored in 100% humidity (0 day, 7 days, 1 month, 3 months) or thermo-cycled (at 7 days, 10,000 cycles, 5-55C°, 30 s). Four 1-mm-thick sections were obtained from each tooth and a push-out test was performed and results compared.
RESULTS: There was a significant difference (ANOVA p<0.05) between the means of push-out test results (MPa ± SE) at different storage periods: 24 hours: 15.2 ± 1.4; 7 days: 16.8 ± 1.5; 1 month: 20.3 ± 1.8; and 3 months: 12.5±2.0. Shear strength was significantly different (Chi-square, p<0.05) at the different slice positions. Apical sections had an increase in strength at 7 days and 1 month, but without statistical significance. The coronal section increased significantly between 0 and 7 days. All sections had reduced strengths between 1 and 3 months, but only the apical sections decreased significantly (ANOVA, p<0.05). There was no significant difference in shear strength between thermally cycled and non-cycled samples (MPa/SE 16.7± 5.9, MPa/SE 16.0 ± 9.3)(p>0.05).
CONCLUSIONS: Long-term storage of test samples affected the bond strength of fiber posts to radicular dentin variably, and should be considered as part of invitro testing.

Keywords: adhesion, fiber post, push-out test, radicular dentin, storage, thermocycling.


 

 

Introduction

Damaged tooth structure get support and retention from various post materials and methods with emphasis on esthetic demands1. Many in vitro tests of fiber post material analyze dentin-cement-post system behavior under proposed clinical situations. These tests were reviewed and reasons for the variability of strength results were indicated2-6: 1. Tests were done on full-root samples (as pull-out and fracture compressive load), or root sections (as micro-push-out or hourglass- and rectangular stick-shaped microtensile testing); 2. As the method of force application differed, values of adhesion reported varied; 3. Pull-out of a whole post from full roots had highest values of bond strength, followed by push-out tests from root sections; 4. Failures and high standard deviations of strengths reduce the reliability of test values. A large number of premature failures (during specimen preparation) occur with hourglass and stick tensile tests; 5. Full root sample testing was dependent on the morphology of radicular dentin; 6. The push-out test of thinperpendicular root sections showed advantages over other testing methods: strength measurements were more consistent, stress distribution was more homogenous (finite element analysis), strength values were less variable, flaws were distributed uniformly and finely throughout the material (this is called a higher Weibull modulus), fewer premature failures were found (confined mainly to root samples not embedded in resin blocks), and finally, there was differentiation of regional differences in bond strength; 7. Moisture, heat and fatigue stresses interplay and reduce the longevity of fiber posts clinically, and were standardized conditions added to samples before testing. These were applied either to whole teeth (which best mimics the clinical situation), but other studies concluded from applying them to dentin powder, isolated dentin sections of coronal dentin, or unsealed samples with an enamel-bonded margin, or bovine teeth; 8. Variability also resulted because tests were applied to different cements, adhesives, posts, dentin substrates and irrigant materials.

The study reported here applies some of these clinical conditions to intact human roots that received fiber bonded posts and were tested by push-out tests with different storage periods and thermocycling. The null-hypothesis states that no difference will be detected after different aging periods and thermocycling of intact roots on the adhesion of fiber posts to radicular dentin at different root section locations.

 

Material and methods

Sample selection

Twenty, caries and crack-free human maxillary central incisors and canines with straight 14-mm-long roots and mature apices were selected. A single canal was verified by 2-angled radiographs.

Ethical approvals

This study was supported by grant no. 149/2009, by Jordan University of Science and Technology, Jordan. The study was independently reviewed and approved by the Research Committee, (Deanship of Research) and the Institutional Research Board (IRB), specialized in humanresearch ethics, (Faculty of medicine).

Sample preparation

Debris were cleaned and the intact teeth were disinfected (30 min, 3% sodium hypochlorite sonic bath; Sultan chemists, 300 Pro. Sonic, Branson Ultrasonics) and stored in thymol solution until the time of testing.

Root Canal Treatment

The crowns were removed 2 mm above the buccal cementoenamel junction (0.5 mm diamond wafering blade; Isomet, Buehler Ltd, Lake Bluff, IL, USA) under copious water cooling. A #15 K-type file was inserted until it could be seen at the apical foramen, and then 1 mm was subtracted from this length to determine the working length. Crowndown technique prepared canals for root canal filling (S1, S2, S3, F1, F2 and F3 ProTaper files on X-smart handpiece; Dentsply Maillefer, Switzerland). Irrigation (1 mL of 1% NaOCl solution; PD, Dentaires SA, Switzerland,) and File-Eze (18% EDTA, Ultradent, South Jordan, UT, USA,) were used between files. A final rinse (5 mL distilled water) and drying (ProTaper Paper Points, Ref A 022W; Dentsply Maillefer, Switzerland) were done, then AH26 resin sealer (Dentsply Deterey, Konstanz, Germany) was applied using a lentulo spiral onto the dry canal.

Cold lateral condensation technique was used to obturate the canals (gutta-percha cone and fine accessory points, ProTaper Gutta Percha Points, Dentsply Maillefer, Switzerland). The coronal part, 2 mm below the orifice, was cleaned and sealed (Cavit-G; 3 M ESPE, Seefeld, Germany). The root tips were immersed in saline solution in a closed container to ensure high humidity. The container was stored in an incubator at a 37°C to allow setting of the sealer for at least 7 days.

Post Space Preparation

Root filling was removed (Gates-Glidden, Gates, Dentsply Maillefer, Switzerland) and a 10-mm post space was prepared, leaving at least 3 mm of the root filling apically (universal burr, Size 2 drill, RelyX™ fiber post kit; t, 3M ESPE, Seefeld, Germany). Posts (RelyX™ Fiber size 2, 3M ESPE, Seefeld, Germany) were cleaned with alcohol, tried for the fit (binding was excluded) and protected from any further contamination. The post space was irrigated with normal saline and then 5 mL of distilled water. Canals were dried with paper points (ProTaper Paper Points, Dentsply Maillefer, Switzerland).

Cementation of the post

The self adhesive resin cement (RelyX™ Unicem, 3M ESPE, Seefeld, Germany,) was activated, mixed (15 s capsule mixer, Italy), and then applied into canal (Elongatin tips, 3M ESPE, Seefeld, Germany) according to the manufacturer's recommendation. The post was seated immediately, slightly twisted, and moderate pressure was applied to hold the post in its position while removing excess and during polymerization (600 mW/cm2, 40 s through the post, 20 s cervical, buccal and lingual surfaces).

Aging and storage

Specimens (n=4, each) were either stored in 100% humidity for different periods (0 days, 7 days, 1 month, 3 months) or thermocycled after 1 week of storage (5-55C°, 10,000 cycles, 30-s soaking, 12-s intervals).

Sectioning

After the storage period, the teeth were mounted in molds of polyester resin (HM 190 unsaturated polyester resins, intermediate petrochemicals industries, Jordan) for the ease of handling and sectioning. The specimens were then sectioned in a Isomet sectioning machine (Buehler Ltd, LakeBluff, IL, USA) with a 0.5 mm diamond wafering blade under copious water cooling, and loss between each slice was measured to be 0.6 mm. Four 1-mm-thick sections were obtained from each tooth and identified as coronal (S1), sub-coronal (S2), epiapical (S3) and apical (S4) (Figure 1).

 

 

 

Bond strength Test

A push-out test was performed in a (Universal Testing Machine, Jinan testing equipment corporation, PRC). The sample mounted on the testing machine on a table with a 3-mm-diameter central opening to allow the escape of the debonded post. An indenter with a diameter of 0.5 mm was used to apply the force on the post in an apico-coronal direction at a crosshead speed of 1 mm/min until the post was dislodged (Figure 2).

 

 

 

Statistical analysis

The retentive strength of the post segment was expressed in MPa, by dividing the load at failure (Newton) by the interfacial area of the post fragment (SL). The latter, being the lateral surface of a truncated cone, was calculated by the formula: SL = ð (R+ r) .[(h2 (R - r) 2] 0.5, Where ð= 3. 14, R: coronal post radius, r: apical post radius and h: root slice thickness. The differences between groups were tested using ANOVA to compare means of tested groups. Chi square and Tukey's HSD test were used to test the results in terms of slice position (p<0.05).

 

Results

Effect of storage period

There was a significant difference between the means of push-out test results at different storage periods (ANOVA p<0.05) (Figure 3). Shear strength was significantly different at 1 and 3 months of storage (Tukey HSD, p<0.05). Immediate or baseline values were considered testing samples at baseline (24 hours of storage) and comparison with 1-week of storage showed that stored samples had a slight increase in test values (0 day: 15.2 ± 1.4; 7 day: 16.8 ± 1.5 MPa/SE). Then the strength increased reaching the maximum at 1 month (20.3 ± 1.8 MPa/SE), then reduced to its lowest value at three months (12.5±2.0 MPa/SE).

 

 

 

Effect of slice position

Shear strength was significantly different at different slice positions (Chi-square, p<0.05). Apical and epiapical sections had an increase in strength at 7 days and 1 month but without statistical significance. Coronal sections had a significant increase in strength between 0 and 7 days. All sections had reduced strengths between 1 and 3 months, but apical section decreased significantly between 1 and 3 months (ANOVA, p<0.05) (Table 1).

 

 

 

Effect of thermal cycling at 1 week

The number of cycles used here are 10,0000 and performed over 10 days. There was no significant difference in shear strength between the two conditions for the whole samples (16.7± 5.9 and 16.0 ± 9.3 MPa/SE)(p>0.05). Subcoronal and apical sections had a non-significant increase, while coronal and epiapical sections had a non-significant decrease of stress values (Table 2).

 

 

 

Discussion

A five-year survival rates for fiber posts are considered satisfactory7-8 and depend on the location of the tooth restored, type of final restoration, presence of proximal contacts from adjacent teeth, presence of a ferrule, remaining coronal structure and occlusal loads9-11. Nevertheless, the failure mode is usually a restorable de-bonding of resin bonded fiber posts, and can be enhanced by using various pretreatment procedures12.

In vitro studies have tried to test dentin bonding behavior from tests applied to dentin sections and not endodontically and post-restored intact teeth. Testing the radicular dentin-bond-cement-post system could be proposed to have different results than testing sections or non-sealed dentin surfaces2-6. Even in this closed system, more standardization is needed.

This study standardized the taper of endodontic preparation, endodontic filling, post space preparation, irrigation, cement, post placement, coronal seal, and light curing for all study samples. Test variables - storage and thermocycling - have been subjected to intact and sealed roots. Then, resin-block addition, around these root samples, aimed to only facilitate the sectioning procedure. Standardizing the cement in this study, and using only RelyX Unicem, was essential. It had similar flexural strength to the conventional resin cements. Even higher strength and modulus of elasticity were evident when light cured. Its low initial pH is characterized by a more rapid rise to neutrality than other cements, regardless to the curing method11. Although criticized, not being able to effectively remove the thick smear layer12, the cement formed sporadic resin tags and hybrid layers, and it had the highest bond strengths when compared to other conventional cements6,13. In addition, this higher chemical interaction between the cement and hydroxyapatite caused no displacement of the resin components across the dentin tubules12. Its strength was not affected if present in thicker cement layers13. And when test samples were examined for failure modes, it was the only one to show an adhesive-cohesive mixed fracture14-16.

The effect of storage

Storage of bonded restorations caused deterioration by infusion of fluid into its matrix, from periodontioum, coronal and apical ends of the restorations. If no peripheral failure is observed in restorations, this leakage is confined to fluids and not to macromolecules and microbial products. Later, a progressive disintegration of the fibrilar network of the collagen occurs in hybrid layer. Matrix metalloproteinase (MMPs) bind to dentin matrix and become activated or come from pulp tissue, odontoblasts, saliva or microbial macromolecules2-6. Further expression of their activity is produced by acidic challenges to dentin tissue during bonding procedures17. Another effect of fluid ingression during storage is the plasticizing of resin bond and cement materials. However, a study, using multiple cements, concluded that 3-month storage of intact samples in deionized water had no influence on the hardness of 3 common post-cements, with the exception of RelyX Unicem. It had a significant increase in the hardness values after storage18.

Comparing strength values at different slice depth

It could be proposed that the restored canal system behaves differently at different depths from the cementoenamel. One difference between layers is the adaptation of the post to canal walls, increasing adhesion forces, and friction, while reducing the low cohesive strength, fluid ingression and flaws relevant to the cement layer19. Apical portions expected to be closer and could be matched in size with the post, by custom-post preparation burs. If a close fit is produced, by post relining for example, higher retention values were obtained than non-relined post, in all thirds, along with disappearance of differences among various depths19. When precise fitting was reached, RelyX Unicem had similar or higher strength values, compared with other cements, after water storage and thermocycling.

In oversized post space, it was second after Build-It, SuperBond and Panavia13,20-21, in comparison with 3 other different cements with thermocycling only. Fracture modes were shown by other cements to be mainly adhesive at the post surface or cohesive for precisely fitting posts and to occur between post and composite, except for RelyX Unicem with cohesive fractures, for the oversized spaces. Finite element model found that indirect and direct restorations produced both strain and stress, off the scale in the middle third of the buccal aspect of the root surface. While minimum values were noticed at the level of both the apical portion of the post and the root apex22. In vitro studies not including the previous clinical conditions have shown the contrary.

Cervical (coronal sections) regions have higher strength values than middle and apical thirds, respectively2-6. The preference of light curing to easily accessible cervical regions could be one reason for this result. RelyX Unicem showed the same result in a previous study23. The reason could have been the amount of non-homogenous cement that was less in the cervical than in the middle and apical levels. The application tip used in this study significantly reduced these failures, but they were still more predominant in apical areas24.

Another problem was that the brush used to coat adhesives accessed cervical regions with more material, leading to better resin tag morphologies25. Using the unique application tip, then inserting the post in it, excluded the formation of air inhibited layers in the cement. Finally, contamination of apical regions with remnants of sealing material is more prominent in apical regions26. This final limitation was excluded from this study as the last 1.4 mm of the post was not included in the sections. This study was persistent with the results of the first group; apical sections were stronger than other sections that gradually decreased up to the coronal. The apical even benefitted to some extent from thermocycling, in the same way as observed in a previous study16, but was significantly affected by the storage, leading it to comply more with the second group27. Studies that have shown a thermocycling effect used a larger number of cycles, 40,000, and were subjected to exposed sections, not intact teeth28.

Within the limitations of this study, it was observed that intact and sealed roots produced different fiber post bonding results in comparison with other studies applying these conditions to dentin sections, powder or un-sealed restorations. Such variation should be taken into account when planning in vitro studies which test and conclude on the behavior of the radicular dentin-cement-post system on the long-term and at different regions within the root.

 

Acknowledgements

This study was supported by grant no. 149/2009, by Jordan University of Science and Technology, Jordan. The study was independently reviewed and approved by the Research Committee, (Deanship of Research) and the Institutional Research Board (IRB), specialized in humanresearch ethics, (Faculty of medicine).

 

References

1. Cagidiaco MC, García-Godoy F, Vichi A, Grandini S, Goracci C, Ferrari M. Placement of fiber prefabricated or custom made posts affects the 3-year survival of endodontically treated premolars. Am J Dent. 2008; 21: 179-84.         [ Links ]

2. Bitter K, Kielbassa AM. Post-endodontic restorations with adhesively luted fiber-reinforced composite post systems: a review. Am J Dent. 2007; 20: 353-60.         [ Links ]

3. Saskalauskaite E, Tam LE, McComb D. Flexural strength, elastic modulus, and pH profile of self-etch resin luting cements. J Prosthodont .2008; 17: 262-8.         [ Links ]

4. Goracci C, Sadek FT, Fabianelli A, Tay FR, Ferrari M. Evaluation of the adhesion of fiber posts to intraradicular dentin. Oper Dent. 2005; 30: 627-35.         [ Links ]

5. Wrbas KT, Kampe MT, Schirrmeister JF, Altenburger MJ, Hellwig E. .[Retention of fiber posts dependent on different resin cements] Schweiz Monatsschr Zahnmed. 2006; 116: 18-24.         [ Links ]

6. Bitter K, Paris S, Pfuertner C, Neumann K, Kielbassa AM. Morphological and bond strength evaluation of different resin cements to root dentin. Eur J Oral Sci. 2009; 117: 326-33.         [ Links ]

7. Signore A, Benedicenti S, Kaitsas V, Barone M, Angiero F, Ravera G. Long-term survival of endodontically treated, maxillary anterior teeth restored with either tapered or parallel-sided glass-fiber posts and full-ceramic crown coverage. J Dent. 2009; 37: 115-21.         [ Links ]

8. Piovesan EM, Demarco FF, Cenci MS, Pereira-Cenci T. Survival rates of endodontically treated teeth restored with fiber-reinforced custom posts and cores: a 97-month study. Int J Prosthodont .2007; 20: 633-9.         [ Links ]

9. Naumann M, Blankenstein F, Kiessling S, Dietrich T. Risk factors for failure of glass fiber-reinforced composite post restorations: a prospective observational clinical study. Eur J Oral Sci. 2005; 113: 519-24.         [ Links ]

10. Naumann M, Reich S, Nothdurft FP, Beuer F, Schirrmeister JF, Dietrich T. Survival of glass fiber post restorations over 5 years. Am J Dent. 2008; 21: 267-72.         [ Links ]

11. Dorriz H, Alikhasi M, Mirfazaelian A, Hooshm, T. Effect of ferrule and bonding on the compressive fracture resistance of post and core restorations. J Contemp Dent Pract. 2009; 10: 1-8.         [ Links ]

12. de Souza Costa CA, Hebling J, Randall RC. Human pulp response to resin cements used to bond inlay restorations. Dent Mater. 2006; 22: 954-62.         [ Links ]

13. Huber L, Cattani-Lorente M, Shaw L, Krejci I, Bouillaguet S. Push-out bond strengths of endodontic posts bonded with different resin-based luting cements. Am J Dent. 2007; 20: 167-72.         [ Links ]

14. Garcia LD, Naves LZ, Correr-Sobrinho L, Consani S, Pires-De-Souza FD. Bond strength of a self-adhesive resinous cement to root dentin irradiated with a 980-nm diode laser. Acta Odontol Scand. 2010; 68: 171-9.         [ Links ]

15. Wrbas KT, Kampe MT, Schirrmeister JF, Altenburger MJ, Hellwig E. .[Retention of fiber posts dependent on different resin cements] Schweiz Monatsschr Zahnmed. 2006; 116: 18-24.         [ Links ]

16. Dimitrouli M, Günay H, Geurtsen W, Lührs AK. Push-out strength of fiber posts depending on the type of root canal filling and resin cement. Clin Oral Investig. 2011; 15: 273-81.         [ Links ]

17. De Munck J, Van den Steen PE, Mine A, Van Landuyt KL, Poitevin A, Opdenakker G, et al. Inhibition of enzymatic degradation of adhesivedentin interfaces. J Dent Res. 2009; 88: 1101-6.         [ Links ]

18. Pedreira AP, Pegoraro LF, de Góes MF, Pegoraro TA, Carvalho RM. Microhardness of resin cements in the intraradicular environment: effects of water storage and softening treament. Dent Mater. 2009; 25: 868-76.         [ Links ]

19. Faria-e-Silva AL, Pedrosa-Filho Cde F, Menezes Mde S, Silveira DM, Martins LR. Effect of relining on fiber post retention to root canal. J Appl Oral Sci. 2009; 17: 600-4.         [ Links ]

20. Schmage P, Pfeiffer P, Pinto E, Platzer U, Nergiz I. Influence of oversized dowel space preparation on the bond strengths of FRC posts. Oper Dent. 2009; 34: 93-101.         [ Links ]

21. Naumann M, Preuss A, Frankenberger R. Load capability of excessively flared teeth restored with fiber-reinforced composite posts and all-ceramic crowns. Oper Dent. 2006; 31: 699-704.         [ Links ]

22. Sorrentino R, Salameh Z, Apicella D, Auriemma T, Zarone F, Apicella A, et al. Three-dimensional finite element analysis of stress and strain distributions in post-and-core treated maxillary central incisors. J Adhes Dent .2007; 9: 527-36.         [ Links ]

23. Boff LL, Grossi ML, Prates LH, Burnett LH, Shinkai RS. Effect of the activation mode of post adhesive cementation on push-out bond strength to root canal dentin. Quintessence Int. 2007; 38: 387-94.         [ Links ]

24. Watzke R, Blunck U, Frankenberger R, Naumann M. Interface homogeneity of adhesively luted glass fiber posts. Dent Mater. 2008; 24: 1512-7.         [ Links ]

25. Vichi A, Grandini S, Ferrari M. Comparison between two clinical procedures for bonding fiber posts into a root canal: a microscopic investigation. J Endod. 2002; 28: 355-60.         [ Links ]

26. Serafino C, Gallina G, Cumbo E, Ferrari M. Surface debris of canal walls after post space preparation in endodontically treated teeth: a scanning electron microscopic study. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2004; 97: 381-7.         [ Links ]

27. Ma S, Nakajima KF, Nishiyama N. Effects of storage temperature on the shelf life of one-step and two-step self-etch adhesives. Oper Dent. 2009; 34: 472-80.         [ Links ]

28. Mazzoni A, Marchesi G, Cadenaro M, Mazzotti G, Di Lenarda R, Ferrari M, et al. Push-out stress for fibre posts luted using different adhesive strategies. Eur J Oral Sci. 2009; 117: 447-53.         [ Links ]

 

 

Correspondence:
Layla Abu-Naba'a
Assistant Professor of Prosthodontics,
Department of substitutive Dental Sciences
Faculty of Dentistry, Taibah University
p.o.box 2898, Madinah Munawarah - KSA
E-mail: laylanabaa@hotmail.com

Received for publication: December 04, 2011
Accepted: April 18, 2012