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Brazilian Journal of Oral Sciences
versão On-line ISSN 1677-3225
Braz. J. Oral Sci. vol.15 no.2 Piracicaba Abr./Jun. 2016
ORIGINAL ARTICLE
Tooth whitening recovers the color of pre-stained composites
Camila Bruscato FarinonI; Giovanna Signori PasqualottoI; Kauani CarraroI; Sinval Adalberto Rodrigues-JuniorI
I Universidade Comunitária da Região de Chapecó – Unochapecó, School of Dentistry, Health Sciences Area, Chapecó, SC, Brazil
ABSTRACT
Aim: To assess the effect of home-whitening on aged and stained composite. Methods: Fifteen disc-shaped specimens (10 mm diameter x 2 mm thick) of Filtek Z350XT, shade A2E were fabricated, polished and embedded in wax, leaving exposed the top surface. The specimens were allocated to three groups (n=5): A – the specimens remained dry; B – conditioning in distilled water and; C – conditioning in distilled water and coffee. They were next subjected to whitening with 10% carbamide peroxide (Whiteness Perfect) for 4 h daily for 1, 2 and 4 weeks. Shade was measured with a spectrophotometer Easyshade and expressed based on the CIE L*a*b* system (ΔE*). Surface roughness was measured with a roughness meter (Ra-μm). Data were analyzed with two-way ANOVA and Tukey's test (α=0.05). Results: Conditioning, grouping and the interaction between both, influenced the ΔE* (p<0.0001), which exceeded the 3.3 threshold for visible color change after conditioning of Group C in black coffee. Whitening for 1 week significantly reduced ΔE* in this group. There were no significant changes in surface roughness. Conclusion: Home-whitening did not alter significantly the color of the nanocomposite, except when it was previously stained. One-week whitening was sufficient to recover color change to an acceptable level.
Keywords: Dentin-Bonding Agents. Dentin. Denture Retention.
Introduction
Tooth whitening has become a major elective demand for dentists in restorative dentistry nowadays1. Usually, the protocol involves advising patients that restored anterior teeth to be whitened would probably have the restoration replaced after tooth whitening, due to discrepancies of color between the recently whitened tooth structure and the restoration2,3. However, it is not uncommon the report of dentists that replacing the restoration was not necessary due to color match with the bleached tooth. Based on this observation, it is hypothesized that the resin composite also undergoes color change during tooth whitening.
Color change in resin composites is well known, either due to intrinsic or extrinsic factors4,5. Intrinsic factors involve the hydrolytic degradation and release of constituents, while extrinsic factors involve the incorporation of pigments by staining substances from the diet4. Hydrolytic degradation is inherent to dimethacrylate-based composites in contact with a wet environment, leading ultimately to a more porous restoration6. Although the increase of porosity of the restoration makes it more prone to staining, water diffusion alone revealed being insufficient to lead to significant, visible color change of a composite restoration4.
Color change in Dentistry has been expressed quantitatively, based on the CIE L*a*b* system. This system provides a summarizing measure (ΔE*) that represents the overall color change, and may be classified into three distinct intervals, considering the ability of the human eye to recognize differences in colors: a ΔE*<1 is considered imperceptible by the eye, while 1 <ΔE*< 3.3 is considered visible only to a skilled person and ΔE* > 3.3 is easily detected by anyone, characterizing the color change of a restoration as clinically unacceptable7,8. Other studies have confirmed the color changing effect of several extrinsic pigments from dietary sources, including grape juice, red wine and coffee4,8-10.
Considering the capillarity of the restorative composite to staining molecules, it seems fair to assume that the hydrogen peroxide (H2O2) resulting from the tooth whiteners would also percolate the material's structure, due to its low molecular weight1. Prior studies have shown some evidence of dental composites whitening3,9, but, the extent to which it is whitened and whether this is sufficient to maintain the restoration following tooth whitening still remains uncertain3. On that basis, this study was designed to verify whether tooth whitening alters the color of a universal nanocomposite and to test the hypothesis that significant color alteration after whitening only occurs when the composite contains acquired extrinsic pigments.
Material and methods
Fifteen disc-shaped specimens with 10 mm diameter and 2 mm thick were built up in a single increment with the universal composite Filtek Z350XT (3M/ESPE, St. Paul, MN, USA), shade A2E. The sample size was calculated by G*Power 3.1.9.2 (Universität Düsseldorf, Düsseldorf, Germany) considering a type I error probability of 0.05, a test power of 95%, one tail and a 3.3 effect size, based on the clinically visible ΔE*7. The specimens were light cured for 20 s using an Ultraled lightcuring unit (Dabi Atlante, Joinville, SC, Brazil) with a minimum irradiance of 600 mW/cm2. Next, the specimens were polished with Diamond Pro (FGM, Joinville, SC, Brazil) sequential discs of decreasing particle sizes at low speed and embedded in white wax, except for the irradiated/polished surface.
The specimens were randomly allocated to three groups (n=5), according to Figure 1.:
Group A: a total 16-day conditioning period prior to whitening involved water degradation and staining of the specimens. Specimens of Group A remained dry during this conditioning period for control purposes.
Group B: the specimens were stored for 9 days in distilled water as a means of imposing hydrolytic degradation. The ageing period was previously determined as required for total water sorption and solubilization of components of a 2-mm thick specimen11. The specimens were kept in distilled water during the next 7-day period.
Group C: these specimens were conditioned for 9 days in distilled water, similar to Group B and then for 7 days in black coffee.
After this, dry, degraded and pigmented specimens were submitted to whitening. Conditioning occurred at 37oC. Following the 16-day conditioning period, the specimens of the three groups were submitted to whitening with 10% carbamide peroxide gel (Whiteness Perfect, FGM) for 4 h daily during 1, 2 and 4 weeks.
Between each conditioning and whitening period the specimen's shade was measured with a spectrophotometer Easyshade (Vita Zahnfabrik, Bad Säckingen, Germany) and expressed based on the CIE L*a*b* system. Color was measured with the spectrophotometer's tip in contact and perpendicular to the specimen's surface. A standard white background was used for all measurements.
The CIE L*a*b* is a three-dimensional color space. L* stands for lightness and varies from 0 (black) to 100 (white). a* varies between green (negative a*) and red (positive a*) and b* varies between blue (negative b*) and yellow (positive b*). Variations between measures throughout different periods generate the deltas (ΔL*, Δa* and Δb*) that, ultimately, yield the ΔE*, which represents the overall color change by the following equation.
Additionally, the surface roughness was measured with a RP-200 roughness meter (Instrutherm, São Paulo, SP, Brazil), by three parallel readings along 4 mm length with a cut-off setting of 0.8 mm.
Data were analyzed with two-way analysis of variance and post-hoc Tukey test (α=0.05).
Results
Variance analysis depicted significant differences in ΔE* due to conditioning (p<0.0001), to grouping (p<0.0001) and to the interaction between both (p<0.0001). Color change was highly significant following conditioning of Group C in black coffee, exceeding the 3.3 threshold for visible color change. Such color change returned to acceptable levels after whitening for 1 week and kept decreasing during the two-week and four-week whitening (Table 1 and Figure 1). This reflected the significant decrease of L* (darkening) and increase of b*(yellowing) following storage of specimens of Group C in black coffee (Figure 1-A and C). Recovery of lightness was observed following one-week whitening, while significant reduction of yellowing occurred only after four-week whitening.
No significant changes in surface roughness were observed with either conditioning or whitening procedures (p>0.05) (Table 1).
Discussion
This study was designed to test the hypothesis that whitening of the composites occurs following a degradation period and the incorporation of extrinsic staining molecules. The degradation period of 9 days was based on prior calculation that considered the time for water sorption in a 2-mm thick specimen11. According to Ferracane12 (2006), clinically placed restorations take up to two months to fully saturate with solvent and leach unconverted components. Other surfaces than the irradiated/polished surface were protected in wax, exposing a single way of percolation through the specimen, similar to what occurs in the clinical situation13.
The susceptibility of the composite to percolation of staining substances is related to its chemical composition, which includes the type of monomer, the type of inorganic filler, the used photoinitiator and the quality of the matrix/filler interface14. It is also modulated by its degree of conversion and network characteristics, since a low degree of conversion leads to degradation of the polymer network and release of byproducts6,12,15,16. The nanocomposite used in this study is recommended to both anterior and posterior restorations and has been characterized elsewhere17, revealing a degree of conversion of 46% on the top surface and of 38% on the bottom surface of a 2 –mm thick specimen. According to the authors, light-scattering effects of the nanoclusters could possibly explain the reduction of degree of conversion in depth in this composite17.
Conditioning in distilled water for nine days generated a ΔE* of 1.2 for all groups. Interestingly, specimens from Group A, which remained dry during this conditioning period, also had the same color change as the ones stored wet. Also, color change was below the 3.3 threshold and within the interval of detection only by a skilled person, meaning that an insignificant color change to the patient's eyes occurs due to hydrolytic degradation4,9.
According to Mendes et al.14 (2012), degradation of the composite is a modulating factor for the uptake of extrinsic pigments. Water sorption and swelling affect the total volume of the restoration and its porosity12,15, enabling the uptake of staining solutions. Conditioning of the specimens from Group C in coffee following degradation, significantly affected ΔE* as a reflection of yellowing (significant increase of b* color coordinate) and darkening (significant reduction of L* color coordinate) (Table 1). Staining would probably be higher if the other surfaces of the specimen were not protected with wax. Even so, an easily perceptible 6.3 ΔE* was observed in Group C as a consequence of conditioning in coffee. As to the specimens from Groups A and B, stored dry and wet, respectively, no significant color change was observed.
The staining potential of coffee has been attributed to the presence of yellow colorants4, which could be confirmed by the significant alteration in the b* axis. Garoushi et al.18(2013) observed that staining solutions not containing yellow colorants presented a lower staining potential. Bagheri et al.19 (2005) noticed that color changes higher than 3.3 are often associated to darkening and yellowing, the L* component being the most significant for color change. In our study, lightness was recovered with one-week whitening, while yellowing took four weeks to reduce significantly. This may be explained by the whitening mechanism of hydrogen peroxide that breaks dark complex pigment molecules into simpler structures with lighter optical characteristics. Therefore, lightness is more quickly achieved than the recovery of yellowing.
Whitening with 10% carbamide peroxide for one week significantly reduced color change from 6.3 to 2.8, and kept reducing it during the subsequent weeks to levels similar to the groups that were not stained (Table 1). The diffusion of H2O2 across enamel and dentin is a well-recognized phenomenon1,20 that occurs based on the Fick's second law of diffusion, considering enamel and dentin as semipermeable membranes and several other variables, such as the surface area, the diffusion coefficient, the concentration and the diffusion distance1. Water sorption by composites is also known to respect the same laws of diffusion12; nevertheless, little is known about the diffusion of H2O2 into previously degraded composite restorations, an issue that demands further research.
Still, one could observe significant whitening of the stained specimens. The whitening mechanism, as it is understood up to now, involves the release of reactive oxygen and its interaction with high molecular weight organic chromophore molecules breaking them into simpler structures with final optical properties that reduce the appearance of stain1,9,21. This was clearly observed in specimens of Group C. However, the lack of significant color change in the specimens from groups A and B reinforces the hypothesis that whitening of composites occurs by removal of extrinsic stain, not to intrinsic color change9,22.
No significant change in surface roughness was observed throughout the treatment conditions, in either group. Expectations were that degradation would lead to solubilization of non-converted monomers, oligomers, catalysts, silane and other by-products, which would expose and dislodge filler particles, increasing surface roughness6,12. This would lead to a more porous surface rising the percolation way for either the staining solution or the whitener. However, neither conditioning in water, nor in the staining solution, nor the whitening procedure were sufficient to increase surface roughness, similar to other studies2,14.
Prior studies have shown that the effect of whiteners on restorative materials are material-dependent3,9, and vary according to the material's composition and microstructure. The constituents of the composite generate a tridimensional microstructure that involves the polymer matrix, the inorganic filler and the matrix/ filler interface17. Hydrolytic degradation of the matrix or the matrix/ filler interface generates percolation paths to both staining molecules and whitener. According to Fontes et al.8(2009), the presence of specific monomers, like TEGDMA, make the composite more prone to degradation. Li and Wang3 (2009) also claim that if the composite is highly cross-linked by high-molecular weight polymer molecules, the whitener may require more time to diffuse through the material.
In our study we focused on a single, extensively studied and well-known nanofill universal composite, which may produce unique interactions with water, the staining solution and specially with the tooth whitener. Therefore, our results should be regarded with caution, not extrapolated to other tooth-coloring materials. This study also attempted to simulate clinical whitening by a home-whitening protocol based on 10% carbamide peroxide and revealed that a 3.35% hydrogen peroxide release1 during a 4-hour/day period was sufficient to recover color change of the composite following staining with coffee. Based on that, one could infer that a clinical in-office whitening protocol based on 35% hydrogen peroxide would easily reach the same results.
The home whitening protocol based on application of 10% carbamide peroxide did not alter significantly the color of the studied nanocomposite, except if it was stained previously with extrinsic pigments, confirming the study hypothesis. One-week whitening was sufficient to recover color change to an acceptable level.
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Corresponding author:
Sinval Adalberto Rodrigues-Junior
Universidade Comunitária da Região de Chapecó
Área de Ciências da Saúde – Caixa Postal 1141
Av. Senador Atílio Fontana, n. 591-E – Efapi
CEP 89809-000 – Chapecó – SC – Brasil
E-mail: rodriguesjunior.sa@unochapeco.edu.br
Received for publication: July 20, 2016
Accepted for publication: November 16, 2016