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Revista Odonto Ciência (Online)

versão On-line ISSN 1980-6523

Rev. odonto ciênc. (Online) vol.25 no.1 Porto Alegre Jan. 2010

 

ORIGINAL ARTICLE

 

Tissue inflammatory response to implantation of calcium hydroxide and iodoform in the back of rats

 

Resposta inflamatória tecidual da implantação de hidróxido de cálcio e iodofórmio em dorso de ratos

 

 

Raul Capp PallottaI; Manoel Eduardo de Lima MachadoII; Norair Salviano dos ReisIII; Guilherme Henrique Rosa MartinsIV; Cleber Keiti NabeshimaIV

IGraduate Course in Endodontics, São Paulo General Hospital, São Paulo, SP, Brazil
IIDepartment of Restorative Dentistry, School of Dentistry, University of São Paulo, São Paulo, SP, Brazil
IIIProfessor, Department of Biological Sciences, Pontifical Catholic University, Campinas, SP, Brazil
IVGraduate Course in Endodontics, Department of Restorative Dentistry, School of Dentistry, University of São Paulo, São Paulo, SP, Brazil

Correspondence

 

 


ABSTRACT

PURPOSE: This study evaluated the inflammatory reaction caused by the implantation of iodoform and calcium hydroxide in the back of rats. These drugs may be used as intracanal dressings to eliminate residual bacteria of the root canal system.
METHODS: Twenty albinic rats (Rattus norvegicus, var Wistar) were divided into four groups: control group 1 (CG1) had normal skin; control group 2 (CG2) had wounded tissue without drugs; in groups 3 and 4, iodoform (IG) and calcium hydroxide (CHG) were inserted into the wounds, respectively. After 3, 5 and 11 days, slices of the implanted areas were macroscopically and microscopically observed regarding to their qualitative and quantitative aspects.
RESULTS: In the macroscopical analysis, the CHG showed a large area of necrosis and swelling, which progressively decreased; in the IG the presence of iodoform surrounded by normal tissue was observed. The qualitative and quantitative histological analysis showed that IG promoted a shorter delay in the inflammatory response than the CHG.
CONCLUSION: The inflammatory reaction for iodoform had a peak period five days after the drug insertion. By comparison, calcium hydroxide showed a very large area of necrosis that could only be partially eliminated after eleven days.

Key words: Calcium hydroxide; Endodontics; inflammation; iodoform


RESUMO

OBJETIVO: O objetivo deste estudo foi avaliar a resposta inflamatória causada pela implantação do iodofórmio ou hidróxido de cálcio em dorso de ratos. Estas drogas podem ser usadas como curativo intracanal para eliminar bactérias residuais do sistema de canal radicular.
METODOLOGIA: Foram utilizados 20 ratos albinos (Rattus norvegicus, var Wistar) e divididos em 4 grupos: grupo controle 1 (CG1) representado por tecido normal íntegro; grupo controle 2 (CG2) com ferida e sem medicação; nos grupos 3 e 4, Iodofórmio (IG) e hidróxido de cálcio (CHG) foram, respectivamente, inseridos nas feridas. Após três, cinco e onze dias, cortes microscópicos das áreas implantadas foram observados macroscópica e microscopicamente quanto a seus aspectos qualitativos e quantitativos.
RESULTADOS: Na análise macroscópica, o CHG mostrou uma grande área de necrose e edema, o que diminuiu progressivamente, no GI, a presença de iodofórmio rodeada por tecido normal foi observado. A análise qualitativa e quantitativa histológica mostrou que IG promoveu um prazo mais curto na resposta inflamatória do que o CHG.
CONCLUSÃO: A reação inflamatória de iodofórmio teve um período de pico de cinco dias após a inserção de drogas. Em comparação, o hidróxido de cálcio mostrou uma grande área de necrose que só poderia ser parcialmente eliminada após onze dias.

Palavras-chave: Hidróxido de cálcio; Endodontia; inflamação; iodofórmio


 

 

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Correspondence:
Raul Capp Pallotta

R. Moreira de Godoi, 664 - 2 andar - cj.07
São Paulo, SP - Brasil 04266 - 060
E-mail: raulcapp@terra.com.br

Received: September 23, 2009
Accepted: December 30, 2009