ORIGINAL ARTICLE

 

Disinfection of irreversible hydrocolloid impressions with sodium hypochlorite steam: Assessment of antimicrobial efficacy

 

Desinfecção de moldes de hidrocolóide irreversível com vapor de hipoclorito de sódio: Avaliação da eficácia antimicrobiana

 

 

Carmem Dolores V. Soares de Moura^I; Walter Leal de Moura^II; Fabiana Mantovani Gomes França^III; Gregório Antonio Soares Martins^IV; Pedro Paulo Feltrim^V; Raquel Virgínia Zanetti^V

^IDepartment of Prosthodontics, Faculty of Dentistry, Federal University of Piauí, Teresina, PI, Brazil
^IIDepartment of Surgery, Faculty of Dentistry, Federal University of Piauí, Teresina, PI, Brazil
^IIIPhD in Dentistry, São Leopoldo Mandic School of Dentistry, Campinas, SP, Brazil
^IVDental School, Federal University of Piauí, Teresina, PI, Brazil
^VSão Leopoldo Mandic School of Dentistry, Campinas, SP, Brazil

Correspondence

 

 

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ABSTRACT

PURPOSE: To evaluate the antimicrobial effectiveness of 2.5% and 5.25% sodium hypochlorite steam to disinfect an irreversible hydrocolloid impressions in Humidifier and Nebulizer Boxes.
METHODS: The study used a total of 80 quadrant impressions of patients, divided into 4 experimental groups of 20 samples each, with respective controls. The impressions were placed in an atmosphere of sodium hypochlorite with 100% relative humidity for 10 min. After disinfection, each impression was immersed in saline solution that was ultrasonically vibrated. Microbiological analysis of the solution was carried out by counting colonies grown in BHI-agar culture medium after 24 hours in an incubator at 37ºC. The data were analyzed using Wilcoxon's t test.
RESULTS: In all groups, it was found that the mean number of colonies in control groups was higher then in the experimental groups (P<0.0001). There was a significant difference between using the Nebulizer Box and the Humidifier Box when 2.5% sodium hypochlorite was used. At a concentration of 5.25% there was no statistical difference between the mean numbers of colonies for the two methods (P>0.01).
CONCLUSION: Sodium hypochlorite at 5.25% can be used for disinfection in the Humidifier Box and Nebulizer Box methods. However, at a 2.5% concentration it is only effective in the Nebulizer box method.

Key words: Disinfection; hypochlorite; irreversible hydrocolloid

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RESUMO

OBJETIVO: avaliar a eficácia antimicrobiana do vapor de hipoclorito de sódio 2,5% e 5,25% na desinfecção de moldes de hidrocolóide irreversível em Caixa Umidificadora e Caixa Nebulizadora.
METODOLOGIA: Utilizaram-se 80 moldes de hemiarcos de pacientes, distribuídos em 4 grupos experimentais de 20 amostras cada, com respectivos controles. Os moldes permaneceram 10 min em atmosfera de hipoclorito de sódio com 100% de umidade relativa. Após desinfecção, cada molde foi imerso em soro fisiológico sob vibração ultrassônica e a análise microbiológica dessa solução foi realizada pela contagem de colônias que cresceram em meio de cultura BHI-ágar após 24h em estufa incubadora a 37ºC. Os dados foram analisados pelo teste t de Wilcoxon.
RESULTADOS: Em todos os grupos observou-se maior número médio de colônias dos grupos controles em relação aos experimentais (P<0,0001). Para 2,5% de hipoclorito de sódio houve diferença estatística significativa entre Caixa Nebulizadora e Caixa Umidificadora. A concentração 5,25% não demonstrou diferença estatística entre os números médios de colônias nos dois métodos utilizados (P>0,01).
CONCLUSÃO: Hipoclorito de sódio 5,25% poderá ser utilizado para desinfecção nos métodos Caixa Umidificadora e Caixa Nebulizadora, no entanto, a concentração 2,5%, só será eficaz quando utilizada no método Caixa Nebulizadora.

Palavras-chave: Desinfecção; hipoclorito de sódio; hidroclóide irreversível

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References

1. leung RL, Schonfeld SE. Gypsum casts as a potencial source of microbial cross-contamination. J Prosthet Dent 1983;49:210-11.         [ Links ]

2. Samaranayake LP, Hunjan M, Jennings KJ. Carriage of oral flora on irreversible hydrocolloid and elastomeric impression materials. J Prosthet Dent 1991;65:244-9.         [ Links ]

3. Brandt RL, Coffey JP, Baker PS. Infection control in a prosthodontic residency program. J Prosthod 1993;2:51-5.         [ Links ]

4. Johnson GH, Drennon DG, Powell GL. Accuracy of elastomeric impressions disinfected by immersion. J Am Dent Assoc 1988;116:525-30.         [ Links ]

5. Jennings KJ, Samaranayake, LP. The persistence of microorganisms on impression materials following disinfection. Int J Prosthod 1991;4:382-7.         [ Links ]

6. Look JO, Clay DJ, Gong K, Messer HH. Preliminary results from disinfection of irreversible hydrocolloid impressions. J Prosthet Dent 1990;63:701-6.         [ Links ]

7. Rueggeberg FA, Beall FE, Kelly MT, Schuster GS. Sodium hypochlorite disinfection of irreversible hydrocolloid impression material. J Prosthet Dent 1992;67:628-31.         [ Links ]

8. Kern M, Rathmer RM, Strub JR. Three-dimensional investigation of accuracy of impression materials after disinfection. J Prosthet Dent 1993;70:449-56.         [ Links ]

9. Schwartz RS, Bradley DV, Hilton TJ et al. Immersion disinfection of irreversible hydrocolloid impressions. Part 1: Microbiology. Int J Prosthodont 1996;7:217-22.         [ Links ]

10. Boden J, Likeman P, Clark R. Some effects of disinfecting solutions on the properties of alginate impression material and dental stone. Eur J Prosthodont Res Dent 2001;9:131-5.         [ Links ]

11. Taylor RL, Wright OS, Maryan C. Disinfection procedures: their effect on the dimensional accuracy and surface quality of irreversível hydrocolóide impression materials and gypsum casts. Dent Mater 2002;18:103-10.         [ Links ]

12. Cottone JA, Molinari JA. Selection for dental practice of chemical disinfectants and sterilants for hepatitis an AIDS. Australian Dent J 1987;32:368-74.         [ Links ]

13. Moimaz SAS, Saliba NA, Corrente JE. Avaliação de quatro índices de higiene oral: aspectos da reprodutibilidade. RPG Rev Pós-Grad 2001;8:46-54.         [ Links ]

14. Mihalow DM, Tinanoff N. The influence of removable partial dentures on the level of Streptococcus mutans in saliva. J Prosthet Dent 1988;59:49-51.         [ Links ]

15. Cohen BI, Pagnillo M, Deutsch AS, Musikant BL. Dimensional accuracy of three different alginate impression materials. J Prosthodont 1995;4:195-9.         [ Links ]

16. Beyerle MP, Hensley DM, Bradley DV, SchwartzbRD, Hilton TJ. Immersion disinfection of irreversible hydrocolloid impressions with sodium hypoclorite. Part I: Microbiology. Int J Prosthodont 1994;7:234-8.         [ Links ]

17. Plummer KD, Wakefield CW. Practical infection control in dental laboratories. Gen Dent 1994;42:545-8.         [ Links ]

18. Fabian TM, Walker SE. Stability of sodium Hypochlorite solutions. Am J Hosp Pharm 1982;39:1016-7.         [ Links ]

19. Trevelyan MR. The prosthetic treatment of hepatitis B antigen positive patients. Brit Dent J 1974;137:63-4.         [ Links ]

20. Jonson GH, Cheillis KD, Gordon GE, Lepe X. Dimensional stability and detail reproduction of irreversible hydrocolloid and elastomeric impressions disinfected by immersion. J Prosthet Dent 1998;79:446-53.         [ Links ]

21. Ivanovski S, Savage NW, Brockhurst PJ, Bird PS. Disinfection of dental stone casts: Antimicrobial effects and physical property alterations. Dent Mater 1995;11:19-23.         [ Links ]

22. Peutzfeldt A, Asmussen E. Effect of disinfecting solutions on surface texture of alginate and elastomeric impressions. Scand J Dent Res 1990; 98: 74-81.         [ Links ]

23. Kaplan BA, Goldstein GR, Boylan R. Effectiveness of a professional formula disinfectant for irreversible hydrocolloid. J Prosthet Dent 1994;71:603-6.         [ Links ]

24. Bergman B, Bergman M, Olsson S. Alginate impression materials, dimensional stability and surface detail sharpness follwing treatment with disinfectant solutions. Swed Dent J 1985;9:255-62.         [ Links ]

25. Dychidala GR. Chlorine and chlorine compounds. In: Block SS, editor. Disinfection, sterilization and preservation. 4^thed. Philadelphia: Lea and Febiger; 1991. p. 131-5.         [ Links ]

 

 

Correspondence:
Carmem Dolores Vilarinho Soares de Moura

Received: November 23, 2009
Accepted: April 15, 2010

 

 

Conflict of Interest Statement: The authors state that there are no financial and personal conflicts of interest that could have inappropriately influenced their work.