Abstract

SOUSA, Alex Paulo Sérvio de; MARTINEZ, Elizabeth Ferreira  and  NAPIMOGA, Marcelo Henrique. Osteoinductive potential of allogeneic human bone blocks in vitro. Rev. Assoc. Paul. Cir. Dent. [online]. 2014, vol.68, n.2, pp. 148-153. ISSN 0004-5276.

The allogenic bone has several comparative advantages in relation to autograft bone, but its use is relatively recent and surrounded by uncertainties regarding the biological/immunological behavior and effectiveness as a biomaterial. Some authors consider the material strictly osteoconductive, while others believe that the material can also possess osteoinductive activity. Thus, we evaluated the osteoinductive potential of allograft bone by analyzing the effects of protein extracts from allograft blocks using human pre-osteoblastic cells. The protein extract from allograft block was incubated in cultures of pre-osteoblast cells in order to verify the cell proliferation potential. Moreover, it was employed the RT-qPCR, Alizarin and Von Kossa staining to evaluate the bone metabolism, as well as, the potential induction of calcium and phosphate nodule formation. Finally, it was quantified the morphogenetic protein BMP-2. It was possible to observe in the experimental group, an increase in the cell proliferation and cellular metabolism, as well as, a significant increase in the formation of nodules of calcium and phosphate in comparison to control group. It was possible to detect amounts of BMP-2 in six of nine total samples tested. It was possible to conclude that the mineralized allografts blocks have in vitro osteoinductive activity on osteoblastic cells.

Keywords : bone transplantation; osteoblasts; bone and bones.